Anti-CUX1 Antibody
Cat# MBS820580-0,2mL
Size : 0.2mL
Marca : MyBiosource
Western blot analysis of CUX1 expression in HeLa (A), NIH3T3 (B) whole cell lysates.
Immunohistochemical analysis of CUX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CUX1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CUX1 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS820580MBS820580 is an antibody targeting CUX1. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to CUX1 More details
Product Overview
Online Datasheet in PDFProduct Name
CUX1, Polyclonal Antibody
Full Product Name
Anti-CUX1 Antibody
Product Gene Name
anti-CUX1 antibody
Product Synonym Names
CUX1; CUTL1; Homeobox protein cut-like 1; CCAAT displacement protein; CDP; Homeobox protein cux-1; CUX1; CUTL1; Protein CASP
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of CUX1 protein.
Preparation and Storage
Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
CUX1 peptide (MBS822452) is used for blocking the activity of CUX1 antibody (MBS820580)
Applications Tested/Suitable for anti-CUX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-CUX1 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)
Immunohistochemistry (IHC) of anti-CUX1 antibody
Immunohistochemical analysis of CUX1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence (IF) of anti-CUX1 antibody
Immunofluorescent analysis of CUX1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Other Notes
Small volumes of anti-CUX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CUX1. It may not necessarily be applicable to this product.
NCBI Official Full Name
protein CASP isoform d
NCBI Official Synonym Full Names
cut-like homeobox 1
NCBI Official Symbol
CUX1
NCBI Official Synonym Symbols
CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
UniProt Protein Name
Homeobox protein cut-like 1
UniProt Synonym Protein Names
CCAAT displacement protein; CDP; Homeobox protein cux-1
Protein Family
Homeobox protein
UniProt Gene Name
CUX1
UniProt Synonym Gene Names
CUTL1; CDP
UniProt Entry Name
CUX1_HUMAN
NCBI Protein Information
protein CASP; cut homolog; homeobox protein cux-1; CCAAT displacement protein; golgi integral membrane protein 6; putative protein product of Nbla10317
NCBI Summary for CUX1
The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]
Show more UniProt Comments for CUX1
CUTL1: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Belongs to the CUT homeobox family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Membrane protein, integral; Transcription factor
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: integral to Golgi membrane; cytosol; nucleus
Molecular Function: sequence-specific DNA binding; chromatin binding
Biological Process: regulation of transcription from RNA polymerase II promoter; intra-Golgi vesicle-mediated transport; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell differentiation; positive regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; kidney development; lung development
Show more Protein type: DNA-binding; Membrane protein, integral; Transcription factor
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: integral to Golgi membrane; cytosol; nucleus
Molecular Function: sequence-specific DNA binding; chromatin binding
Biological Process: regulation of transcription from RNA polymerase II promoter; intra-Golgi vesicle-mediated transport; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell differentiation; positive regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; kidney development; lung development
Research Articles on CUX1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-CUX1 antibody
| Reference Product | PubMed Publications |
|---|---|
| TLE4 antibody | >2 publications with CUX1 and TLE4 |
| BCR antibody | >1 publications with CUX1 and BCR |
Pathways associated with anti-CUX1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Disease Pathway antibodies | Disease Pathway Diagram |
| Signaling By FGFR In Disease Pathway antibodies | Signaling By FGFR In Disease Pathway Diagram |
| Signaling By FGFR Mutants Pathway antibodies | Signaling By FGFR Mutants Pathway Diagram |
| Signaling By FGFR1 Fusion Mutants Pathway antibodies | Signaling By FGFR1 Fusion Mutants Pathway Diagram |
| Signaling By FGFR1 Mutants Pathway antibodies | Signaling By FGFR1 Mutants Pathway Diagram |
Diseases associated with anti-CUX1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Breast Neoplasms Antibodies | >11 publications with CUX1 and Breast Neoplasms |
| Disease Models, Animal Antibodies | >10 publications with CUX1 and Disease Models, Animal |
| Kidney Diseases Antibodies | >9 publications with CUX1 and Kidney Diseases |
| Death Antibodies | >7 publications with CUX1 and Death |
| Uterine Neoplasms Antibodies | >7 publications with CUX1 and Uterine Neoplasms |
| Nervous System Diseases Antibodies | >7 publications with CUX1 and Nervous System Diseases |
| Carcinogenesis Antibodies | >6 publications with CUX1 and Carcinogenesis |
| Inflammation Antibodies | >5 publications with CUX1 and Inflammation |
| Adenocarcinoma Antibodies | >5 publications with CUX1 and Adenocarcinoma |
| Necrosis Antibodies | >4 publications with CUX1 and Necrosis |
Organs/Tissues associated with anti-CUX1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >25 publications with CUX1 and Brain |
| Blood Antibodies | >16 publications with CUX1 and Blood |
| Kidney Antibodies | >11 publications with CUX1 and Kidney |
| Nerve Antibodies | >9 publications with CUX1 and Nerve |
| Embryonic Tissue Antibodies | >9 publications with CUX1 and Embryonic Tissue |
| Lung Antibodies | >7 publications with CUX1 and Lung |
| Bone Antibodies | >5 publications with CUX1 and Bone |
| Liver Antibodies | >5 publications with CUX1 and Liver |
| Bone Marrow Antibodies | >3 publications with CUX1 and Bone Marrow |
| Muscle Antibodies | >3 publications with CUX1 and Muscle |
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