Anti-DNMT1 Antibody (5Y204)
Cat# NB-64-116517-50uL
Size : 50uL
Marca : Neo Biotech
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Anti-DNMT1 Antibody (5Y204)
(Synonyms: MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM) Copy Product InfoSynonyms: MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Catalog No. TMAC-01168 Copy Product Info
Anti-DNMT1 Antibody (5Y204) is a Rabbit antibody targeting DNMT1. Anti-DNMT1 Antibody (5Y204) can be used in ICC/IF,IHC,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
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| Description | Anti-DNMT1 Antibody (5Y204) is a Rabbit antibody targeting DNMT1. Anti-DNMT1 Antibody (5Y204) can be used in ICC/IF,IHC,WB. |
| Synonyms | MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM |
| Ig Type | IgG |
| Clone | 5Y204 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Dnmt1 on HepG2 cells lysates using anti-Dnmt1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Dnmt1 antibody. Counter stained with hematoxylin. 3. ICC staining Dnmt1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Dnmt1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Dnmt1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.  , , , , |
| Application | ICC/IF IHC WB |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID | P26358 |
| Molecular Weight | Theoretical: 183 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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