General Information
| Short Description | Rabbit polyclonal anti-Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (401-450 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human ENPP2 at the amino acid range 401-450 |
| Immunogen Region | 401-450 aa |
| Specificity | Autotaxin Polyclonal Antibody detects endogenous levels of Autotaxin protein. |
Additional Info
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. |
| Function | Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Its major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | AutotaxinEctonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2Extracellular Lysophospholipase DLysopld |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Autotaxin antibodyAnti-Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 antibodyAnti-E-Npp 2 antibodyAnti-Extracellular Lysophospholipase D antibodyAnti-Lysopld antibodyAnti-ENPP2 antibodyAnti-ATX antibodyAnti-PDNP2 antibody |
Information sourced from Uniprot.org