Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Anti-HSP70 Antibody

Anti-HSP70 Antibody

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Cat# CPA9192-30ul

Size : 30ul

Marca : Cohesion Biosciences


  • Western blot analysis of HSP70 expression in Hela (A), mouse brain (B) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of HSP70 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of HSP70 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-HSP70 Antibody
Cat No:CPA9192
Source:Mouse
Reactivity:H, M, R
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Mouse monoclonal antibody to HSP70
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence of human HSP70. The exact sequence is proprietary.
Purification：Affinity chromatography
Clonality：Monoclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/1000 - 1/2000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Gene Symbol：HSPA1L; HSPA1A
Alternative Names：HSPA1L; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 1-Hom; HSP70-Hom; HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Entrez Gene (Human)： 3305; 3303; 3304;
Entrez Gene (Mouse)： 15482; 193740;
Entrez Gene (Rat)： 24963; 24472;
SwissProt (Human)： P34931; P08107;
SwissProt (Mouse)： P16627; Q61696;
SwissProt (Rat)： P55063; Q07439;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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