Mouse anti-Human Kappa Light Chain Antibody
Anti-Kappa Light Chain Antibody
Anti-Kappa Light Chain Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg |
| Species | Human |
| Specificity | Does not react with rat. Other species not tested. |
| Host Species | Mouse |
| Accession Number | P01601|P01834 |
| Gene Id | 3514 |
| Gene Symbols | IGKC, IGKV1D-16 |
| Protein Name / Synonyms | Immunoglobulin kappa variable 1D-16 (Ig kappa chain V-I region HK146) (Ig kappa chain V-I region HK189) (Fragment), Immunoglobulin kappa constant (Ig kappa chain C region) (Ig kappa chain C region AG) (Ig kappa chain C region CUM) (Ig kappa chain C region EU) (Ig kappa chain C region OU) (Ig kappa chain C region ROY) (Ig kappa chain C region TI) |
| Immunogen | Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | FACS: 0.5-2.0ug/million cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml IHC (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cell surface, cytoplasmic and secreted |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Storage
Store the Kappa Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.