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> Anti-N Cadherin Antibody (3O810)

Anti-N Cadherin Antibody (3O810)

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Cat# NB-64-159136-100uL

Size : 100uL

Marca : Neo Biotech


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Anti-N Cadherin Antibody (3O810)

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Catalog No. TMAB-09199 Copy Product Info
Anti-N Cadherin Antibody (3O810) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3O810) can be used in WB,IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-N Cadherin Antibody (3O810) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3O810) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
3O810
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with N Cadherin monoclonal antibody, unconjugated (TMAB-09199) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
7. 25 μg total protein per lane of various lysates (see on figure) probed with N Cadherin monoclonal antibody, unconjugated (TMAB-09199) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
8. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
10. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with N Cadherin Monoclonal Antibody, Unconjugated (TMAB-09199) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Antigen Details
Immunogen
A synthesized peptide: human Cadherin 2
Antigen Species
Human
Gene Name
CDH2
Gene ID
1000
Protein Name
Cadherin-2
Uniprot ID
P19022
Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Chemical Properties
Molecular WeightTheoretical: 100 kDa. Actual: 130 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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