Mouse anti-Human/Mouse/Rat/Guinea pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken NF-H Antibody (phospho) [IHC only]
Anti-NF-H Antibody (phospho)
Anti-NF-H Antibody (phospho)
Contact Us for Bulk Request
Product Description
Specifications
| Size | 7 mL |
| Species | Human, Mouse, Rat |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P12036 |
| Gene Id | 4744 |
| Gene Symbols | NEFH, KIAA0845, NFH |
| Protein Name / Synonyms | Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein) |
| Immunogen | Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho). |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide, *For IHC use only* |
| Recommended Applications | Prediluted format : incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
This mAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
Storage
Store the NF-H antibody (phospho) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.