Anti-Phospho-HDAC2-Ser394 antibody (330-410)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	HDAC2
Gene ID:	3066
Uniprot ID:	HDAC2_HUMAN
Immunogen Region:	360-409 aa
Specificity:	Phospho-HDAC2 (S394) Polyclonal Antibody detects endogenous levels of HDAC2 protein only when phosphorylated at S394.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human HDAC2 around the phosphorylation site of Ser394 at the amino acid range 360-409

Post Translational Modifications	S-nitrosylated by GAPDH. In neurons, S-nitrosylation at Cys-262 and Cys-274 does not affect enzyme activity, but induces HDAC2 release from chromatin. This in turn increases acetylation of histones surrounding neurotrophin-dependent gene promoters and promotes their transcription. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
Function	Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively.
Protein Name	Histone Deacetylase 2 Hd2 Protein Deacylase Hdac2
Database Links	Reactome: R-HSA-193670 Reactome: R-HSA-2122947 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-3214815 Reactome: R-HSA-350054 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-4551638 Reactome: R-HSA-6804758 Reactome: R-HSA-73762 Reactome: R-HSA-8943724 Reactome: R-HSA-9022692 Reactome: R-HSA-9022699 Reactome: R-HSA-9615017 Reactome: R-HSA-9619665 Reactome: R-HSA-9679191 Reactome: R-HSA-9701898 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Cytoplasm
Alternative Antibody Names	Anti-Histone Deacetylase 2 antibody Anti-Hd2 antibody Anti-Protein Deacylase Hdac2 antibody Anti-HDAC2 antibody

Information sourced from Uniprot.org

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