Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-C5) - HRP
Cat# LT7032-50
Size : 50ug
Marca : Leinco Technologies
AntiSARSCoV2 Nucleocapsid (N) (Clone NP1C5) – HRP
AntiSARSCoV2 Nucleocapsid (N) (Clone NP1C5) – HRP
Product No.: LT7032
Product No.LT7032 Clone NP1C5 Target SARSCoV2 Nucleocapsid (N) Product Type Recombinant Monoclonal Antibody Alternate Names COV2NP1C5, SARSCoV2 Nucleocapsid, SARSCoV2 Nucleoprotein, Protein N, SARSCoV N Protein Isotype Human IgG1 Applications ELISA |
Antibody DetailsProduct DetailsReactive Species SARSCoV2 ⋅ Virus Expression Host HEK293 Cells Immunogen SARSCoV2 Nucleocapsid (N) Protein Product Concentration 0.5 mg/ml Formulation This HRPconjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase) Storage and Handling This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 28°C. Do not freeze. Country of Origin USA Shipping Ships Overnight on Blue Ice RRIDAB_2893993 Applications and Recommended Usage? Quality Tested by Leinco ELISA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity AntiSARSCoV2 Nucleocapsid, clone NP1C5, specifically targets an epitope on the SARSCoV2 nucleocapsid protein. Furthermore, it is reported to bind to the RNA binding domain of the N protein. Background Coronavirus disease 2019 (COVID19) is caused by severe acute respiratory syndrome coronavirus 2 (SARSCoV2). SARSCoV2 belongs to the Coronaviridae family, and its singlestranded, positivesense RNA genome shares 79.6% identity with SARSCoV1. The spike (S), envelope (E), membrane (M), and nucleocapsid proteins (N) are four essential structural proteins of SARSCoV22. The 46 kDa N protein is highly conserved and shares 90% homology with SARSCoV3. Similar to SARSCoV, SARSCoV2 has an Nterminal (NTD) and Cterminal domain (CTD), linked by a linker region. The NTD binds to RNA, while the CTD selfoligomerizes4,5, aiding viral genome packaging into a helical ribonucleoprotein complex6. The N protein also participates in viral transcription, replication, and modulation of cell signaling pathways7,8. Some vaccine and diagnostic assays9 have focused on the N protein as it is highly expressed during infection and activates antibodies3,10 and memory T cells11,12, found in convalescent sera. The Nprotein also evades the innate immune system by inhibiting RNAi13, identifying it as a potential therapeutic target. Antigen Distribution The nucleocapsid protein is expressed in the internal nucleocapsid of SARSCoV2. NCBI Gene Bank ID Research Area COVID19 . Infectious Disease . Seasonal and Respiratory Infections . Viral . IVD Raw Material References & Citations1. Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020. 2. Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020. 3. Guo L., Ren L., Yang S., et al. Clinical Infectious Diseases: an Official Publication of the Infectious Diseases Society of America. 2020. 4. Kang S, Yang M, Hong Z, et al. Acta Pharm Sin B. 10.1016/j.apsb.2020.04.009. 2020. 5. Chang CK, Sue SC, Yu TH, et al. J Biomed Sci. 13(1):5972. 2006. 6. Hsieh PK, Chang SC, Huang CC, et al. J Virol. 79(22):1384813855. 2005. 7. Surjit M, Lal SK. Infect Genet Evol. 8(4):397405. 2008. 8. Hurst KR, Ye R, Goebel SJ, Jayaraman P, Masters PS. J Virol. 84(19):1027610288. 2010. 9. Liu L, Liu W, Zheng Y, et al. Microbes Infect. 22(45):206211. 2020. 10. To K.K., Tsang O.T., Leung W.S., et al. Lancet Infect. Dis. 2020. 11. Grifoni A., Weiskopf D., Ramirez S.I., et al. Cell. 2020. 12. Ni L, Ye F, Cheng ML, et al. Immunity. 52(6):971977.e3. 2020. 13. Mu J, Xu J, Zhang L, et al. Sci China Life Sci. 14. 2020. |