Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

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Cat# CoV2-RBT

Size : 100uL

Marca : Phosphosolutions

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Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

Our SARS-CoV-2 S-Protein ACE2 Binding Domain rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects and is antigen affinity purified. It is great for use in WB, ICC.

Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with B-tubulin as a loading control.

Immunofluorescence of SARS-CoV-2 transfected HEK293 cells showing specific staining of SARS-CoV-2 in red. Nuclei are visualized in blue with DAPI and B-tubulin co-staining is shown in green.

Western blot showing specific labeling of SARS-CoV-2 at ~ 25k in green in lane 3. Lane 4 shows GFP-tagged SARS-CoV-2 at ~ 50k in green and lane 2 shows no CoV-2 staining in untransfected cells. The red bands show staining with B-tubulin as a loading control.

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Species Reactivity

SARS-CoV2

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: CoV2-RBT

Ships: 1-2 business days

Product Details

Target

SARS-CoV-2 S-Protein ACE2 Binding Domain

Scientific Background

The novel SARS-coronavirus 2 (SARS-CoV-2) which causes the disease COVID-19 has been shown to utilize the SARS-CoV receptor ACE2 for entry into human cells (Hoffman, M. et al., Cell 2020). The entry of a coronavirus into host cells is mediated by the viral surface-anchored transmembrane spike (S) glycoprotein which is composed of two functional subunits, S1 which binds the receptor and S2 which fuses the membrane (Walls, AC et.al., Cell 2020). S1 contains a receptor-binding domain (RBD) which specifically recognizes ACE2 as its receptor (Wan, Y. et al., 2020).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

Molecular Weight

25 kDa or 142 kDa full-length

Immunogen

Recombinant SARS-CoV-2 S-Protein construct containing the ACE2 binding domain expressed in and purified from E. coli.

Immunogen Species

SARS-CoV2

Species Reactivity

SARS-CoV2

Antibody Registry ID

AB_2868515

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

The antibody was purified over a column to which the protein antigen was coupled.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:2000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~25 kDa ACE2 binding domain construct of SARS-CoV-2 S protein. Full-length CoV-2 S protein is ~142 kDa.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

UniProt ID

P0DTC2

Shipping

Blue Ice

Western Blot Protocol: Download

Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

Cat# CoV2-RBT

Marca : Phosphosolutions

Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody

SKU: CoV2-RBT

Product Details

Bulk Order Anti-SARS-CoV-2 S-Protein ACE2 Binding Domain Antibody