Anti-Vinculin (RABBIT) Antibody

Cat# 600-401-FW5

Size : 100ug

Marca : Rockland Immunochemicals

Contatta il distributore locale :


Telefono : +1 850 650 7790

Background

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).

Expand Background

Specifications for Vinculin Antibody

Product Details

Anti-Vinculin (RABBIT) Antibody - 600-401-FW5
Vinculin Antibody , MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
Rabbit
Polyclonal
IgG

Target Details

VCL  - View All VCL Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Vinculin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the C-terminus of human Vinculin.
Anti-Vinculin Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.
UniProtKB - P18206
GeneID - 7414
NCBI - NP_003364.1

Application Details

ELISA, IF, IHC, WB
Anti-Vinculin Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 124 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Invalid lot number

Search

If you need help finding your CoA, contact us

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Vinculin Antibody

Related Resources

Si può anche essere interessati nei seguenti prodotti:



Cat#
Descrizione
Cond.
Priced
ASC12055
 100ug 
ASC11704
 100ug