Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Anti-Wnt16 Polyclonal Antibody

Anti-Wnt16 Polyclonal Antibody

Stampa Stampa
Entra

Cat# NB-64-164103-100uL

Size : 100uL

Marca : Neo Biotech


Remove All

Anti-Wnt16 Polyclonal Antibody

Copy Product Info

Synonyms:

Catalog No. TMAB-14186 Copy Product Info
Anti-Wnt16 Polyclonal Antibody is a Rabbit antibody targeting Wnt16. Anti-Wnt16 Polyclonal Antibody can be used in WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Wnt16 Polyclonal Antibody is a Rabbit antibody targeting Wnt16. Anti-Wnt16 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Pig,Cow,Rabbit)
Verified Activity
Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-Wnt16 (TMAB-14186) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 56 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.extracellular space, extracellular matrix.
Tissue SpecificityIsoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14, rather than Wnt-15, is preferentially expressed in various types of human cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only in pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemia patients carrying the E2A-Pbx1 hybrid gene. Thus, Wnt-16 is a downstream target of E2A-Pbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t(1;19) pre-B acute lymphoblastoid leukemias.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Wnt16
Antigen Species
Human
Gene Name
WNT16
Gene ID
51384
Protein Name
Protein Wnt-16
Uniprot ID
Q9UBV4
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters
Chemical Properties
Molecular WeightTheoretical: 37 kDa. Actual: 56 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Dilution Calculator
  • Reconstitution Calculator
Related Tags: Anti-Wnt16 Polyclonal Antibody molecular weight