- More Files
Western Blot (Cell lysate)
Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).
Conversion of pATF6alpha (P) to pATF6alpha (N) in DTT- or tunicamycin-treated cells. Tm: 2 ug/mL tunicamycin (inhibitor of N-glycosylation). DTT : 1mM dithiothreitol (reducing reagent). The asterisk denotes an unglycosylated form of pATF6alpha (P).
ATF6alpha is constitutively expressed as pATF6alpha (P) (~90-kDa protein), and converted to pATF6alpha (N) (>50-kDa protein) in ER-stressed cells.
Immunoprecipitation
IP-Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).
- Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ATF6.
Immunogen
Recombinant His fusion protein corresponding to N-terminus human ATF6.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot (Cell lysate)
Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).
Conversion of pATF6alpha (P) to pATF6alpha (N) in DTT- or tunicamycin-treated cells. Tm: 2 ug/mL tunicamycin (inhibitor of N-glycosylation). DTT : 1mM dithiothreitol (reducing reagent). The asterisk denotes an unglycosylated form of pATF6alpha (P).
ATF6alpha is constitutively expressed as pATF6alpha (P) (~90-kDa protein), and converted to pATF6alpha (N) (>50-kDa protein) in ER-stressed cells.Immunoprecipitation
IP-Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762). - Gene Info — ATF6
Entrez GeneID
22926Protein Accession#
P18850Gene Name
ATF6
Gene Alias
ATF6A
Gene Description
activating transcription factor 6
Omim ID
605537Gene Ontology
HyperlinkGene Summary
ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM
Other Designations
OTTHUMP00000025585|activating transcription factor 6 alpha
- Interactome
- Disease
- Publication Reference
- The p38-activated ER stress-ATF6α axis mediates cellular senescence.
Kim HS, Kim Y, Lim MJ, Park YG, Park SI, Sohn J.
FASEB Journal 2018 Sep; fj201800836R.
Application：WB-Tr, Human, MCF-7 cells.
- Identification and characterization of two novel spliced genes located in the orf47-orf46-orf45 gene locus of Kaposi's sarcoma-associated herpesvirus.
Chang PJ, Hung CH, Wang SS, Tsai PH, Shih YJ, Chen LY, Huang HY, Wei LH, Yen JB, Lin CL, Chen LW.
Journal of Virology 2014 Sep; 88(17):10092.
Application：WB-Tr, Human, 293T cells.
- Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1.
Yamamoto K, Sato T, Matsui T, Sato M, Okada T, Yoshida H, Harada A, Mori K.
Developmental Cell 2007 Sep; 13(3):365.
Application：IP, WB, Human, HeLa cells.
- Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Haze K, Yoshida H, Yanagi H, Yura T, Mori K.
Molecular Biology of the Cell 1999 Nov; 10(11):3787.
- Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
Hai TW, Liu F, Coukos WJ, Green MR.
Genes & Development 1989 Dec; 3(12B):2083.
- The p38-activated ER stress-ATF6α axis mediates cellular senescence.
