Biotinylated Human Fibronectin (ED-B domain, N-Avi-6His)
Cat# CR42-100ug
Size : 100ug
Marca : Novoprotein
Description Biotinylated Recombinant Human Fibronectin is produced by our E.coli expression system and the target gene encoding Glu1266-Thr1356 is expressed with a Avi, 6His tag at the N-terminus.
Accession P02751-15
Known As Fibronectin; FN1
Mol Mass 13.4 KDa
APMol Mass 15 KDa, reducing conditions
Formulation Description Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin < 1 EU/µg as determined by LAL test.
Purity-SDS-PAGE 
Greater than 95% as determined by reducing SDS-PAGE. (QC verified) Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage - Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping - The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
Background Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. Similar to integrins, fibronectin binds extracellular matrix components such as collagen, fibrin, and heparan sulfate proteoglycans. Fibronectin plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.