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> Bovine IL-10 ELISA Kit

Bovine IL-10 ELISA Kit

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Cat# ELB-IL10-2

Size : 2PlateKit

Marca : RayBiotech


25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

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Bovine IL-10 ELISA Kit

Bovine IL-10 ELISA Kit

RayBio® Bovine IL-10 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesBovine (cow)
Sensitivity
420 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
420 pg/ml -7000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: Bovine ANG-1, CD40 Ligand, Decorin, IFN beta, IL-1 beta, IL17A, IL-18, LIF, RANTES (CCL5).
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
P43480
Gene Id
281246
Gene Symbols
IL10
Protein Name / Synonyms
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaInflammation
Shipping TypeBlue ice
Storage-20°C

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Bovine IL-10 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-10 (IL10), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELB-IL10.pdf)
SDS (ELB-IL10.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Effects of gut barrier dysfunction during a viral respiratory disease challenge on immune function of feedlot beef calves

    Species:
    Bovine
    Sample Type:
    Serum (feedlot calves, BHV-1 challenge)
    Year:
    2026
    Journal Name:
    Journal of Animal Science
    PubMed ID:
    41968102

  • Clinical study and the diagnosis of lumpy skin disease in cattle using genomic, immunological, and pathological indicators

    Species:
    Bovine
    Sample Type:
    Serum (LSDV-infected vs control cattle)
    Year:
    2026
    Journal Name:
    Frontiers in Veterinary Science
    PubMed ID:
    42109875