Size : 1mLx10mM(inDMSO)
Synonyms:
| Description | Bromophenol Blue, an acid phthalein dye, is utilized both as a tracking dye for electrophoresis and as a pH indicator with a transition range of pH 3 to 4.6. |
| In vitro | Instructions 1. Solvent selection: Bromophenol blue is usually dissolved in water, but can also be dissolved in organic solvents such as ethanol or DMSO depending on the specific experimental needs. 2. Electrophoresis tracking: Bromophenol blue is often used as a tracking dye in gel electrophoresis. It migrates with the sample and helps monitor the progress of the electrophoretic separation process. Concentration: Typical concentrations are 0.01% to 0.1% (w/v). Directions: The dye is mixed with the sample and loaded into the gel. 3. pH indicator: Bromophenol blue is a pH indicator that can change in the pH range of 3.0 to 4.6. It is yellow at low pH and blue at high pH. Directions: Prepare a diluted Bromophenol blue solution for monitoring pH changes in buffers or solutions. Protein detection: Bromophenol blue can be used to detect proteins by binding to the protein, which causes a color change that can be detected by spectrophotometry. 4. Absorption peak: When bound to proteins, Bromophenol blue produces a strong absorption peak at 610 nm. Sensors for chemical detection: Bromophenol blue can also be used as a sensor to detect a variety of compounds such as ammonia, drugs, proteins, and amino acids because it undergoes a color change when bound to these substances. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 669.96 |
| Formula | C19H10Br4O5S |
| Cas No. | 115-39-9 |
| Smiles | O=S1(=O)OC(C=2C1=CC=CC2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC(Br)=C(O)C(Br)=C4 |
| Relative Density. | 0.954 g/cm3 at 20℃ |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 11 mg/mL (16.42 mM), Sonication is recommended. DMSO: 147 mg/mL (219.42 mM), Sonication is recommended.  | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (4.93 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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