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> BRSK1 Antibody

BRSK1 Antibody

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Cat# ASM10546-A680-100

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Marca : Abcepta

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  • ICC/IF - BRSK1 Antibody ASM10546
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BRSK1 Polyclonal Antibody (ASM10546). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BRSK1 Polyclonal Antibody (ASM10546) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BRSK1 Antibody (D) Composite.
  • WB - BRSK1 Antibody ASM10546
    Western blot analysis of Mouse Brain cell lysates showing detection of ~85.1 kDa BRSK1 protein using Rabbit Anti-BRSK1 Polyclonal Antibody (ASM10546). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BRSK1 Polyclonal Antibody (ASM10546) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85.1 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q8TDC3
Other Accession NP_115806.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human BRSK1 Polyclonal
Target/Specificity Detects ~70 kDa.
Other Names Brain-selective kinase 1 Antibody, Brain-specific serine/threonine-protein kinase 1 Antibody, hSAD1 Antibody, BR serine/threonine-protein kinase 1 Antibody, SAD1 Antibody, BRSK1 Antibody, Synapses of Amphids Defective homolog 1 Antibody, KIAA1811 Antibody, SAD1 homolog Antibody, Serine/threonine-protein kinase BRSK1 Antibody, SADB Antibody, Serine/threonine-protein kinase SAD-B Antibody
Immunogen Synthetic peptide from the mid-protein of Human BRSK1
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-686 was sufficient for detection of BRSK1 in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus | Cytoplasm | Cytoskeleton | Microtubule Organizing Center | Centrosome | Cell Junction | Synapse