CANFAST™ Transfection Reagent
Cat# T0083
Size : 1mL
Marca : Canvax Biotech
CANFAST™ Transfection Reagent
High-efficiency, non-toxic DNA transfection reagent with fast protocol and reproducible results in adherent and suspension cells
CanFast™ Transfection Reagent from Canvax™ is a ready-to-use reagent developed for the efficient delivery of DNA or RNA into a wide range of eukaryotic cells. It allows a fast and simple transfection procedure with high reproducibility and minimal cytotoxicity.
This reagent is suitable for transient or stable transfection experiments in various cell lines. Its optimized formulation ensures reliable performance and consistent gene expression in molecular and cell biology research.
With proven performance across a broad range of mammalian cell types, CANFAST™ ensures cost-effective and reliable results for gene expression, co-transfection, and primary cell studies.
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Detailed information:
Advantages & Features
- High transfection efficiency in most mammalian cell lines.
- Non-toxic: cell viability consistently >90%.
- Serum-compatible: works in complete media.
- Time-saving protocol: successful transfections in 24 minutes.
- Broad applicability: adherent, suspension, stable and transient transfections.
- Cost-effective: requires minimal DNA and no media change.
Specifications
Includes
-1 mL CANFAST™ Transfection Reagent (1 µg/µL)
-10 µL GFP-Plasmid Transfection Control (1 µg/µL)
(1 mL = 250 – 500 transfections)
Download documentation
Applications
- High-throughput DNA transfection.
- Stable & transient transfection assays.
- Co-transfection experiments.
- Primary cell transfection.
- Adherent and suspension cell lines.
- Gene expression & cellular analysis studies.
Tables & Figures
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Quality Control
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Transfection efficiency validated with GFP-expression plasmid in Jurkat cells (>50%).
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Lot-to-lot consistency ensured.
Advice
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Optimize DNA:reagent ratio for each cell type.
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Use serum-containing media for improved cell survival.
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Gently mix, do not vortex, to preserve reagent activity.
Storage, Shipping & Guarantee
- Shipped in: Gel pack
- Storage: 4 ºC.
Citations
- Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
- Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Caballero-Villarraso, J., … & Paz-Rojas, E. (2022). Development of a High-Throughput Calcium Mobilization Assay for CCR6 Receptor Coupled to Hydrolase Activity Readout. Biomedicines, 10(2), 422.
- Gómez-Melero, S., García-Maceira, F. I., García-Maceira, T., Luna-Guerrero, V., Montero-Peñalvo, G., Túnez-Fiñana, I., & Paz-Rojas, E. (2021). Amino Terminal Recognition by a CCR6 Chemokine Receptor Antibody Blocks CCL20 Signaling and IL-17 Expression via β-arrestin.
- Yago-Ibáñez, J., García-Pastor, C., Lucio-Cazaña, F. J., & Fernández-Martínez, A. B. (2020). Retinoic acid receptor-beta prevents cisplatin-induced proximal tubular cell death. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 165795.
- Pilná, H. (2019). Vliv interferon regulujícího faktoru 3 na imunitní odpověď proti viru vakcínie v atopickém organismu.
- Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging Cell.
- Martínez-Miguel, P., Valdivielso, J. M., Medrano-Andrés, D., Román-García, P., Cano-Peñalver, J. L., Rodríguez-Puyol, M., … & López-Ongil, S. (2014). The active form of vitamin D, calcitriol, induces a complex dual upregulation of endothelin and nitric oxide in cultured endothelial cells. American Journal of Physiology-Endocrinology and Metabolism, 307(12), E1085-E1096.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Transfection reagent, DNA transfection reagent, Cell transfection reagent, Gene delivery reagent, Lipid-based transfection reagent
Also known as:
- Spanish: Reactivo de transfección, Reactivo para transfección de ADN, Reactivo de entrega génica
- French: Réactif de transfection, Réactif de transfection ADN, Réactif de transfert génique
- German: Transfektionsreagenz, Reagenz für DNA-Transfektion, Genübertragungsreagenz
- Italian: Reagente di trasfezione, Reagente per trasfezione DNA, Reagente per trasferimento genico
FAQs
Q: How many transfections can 1 mL provide?
A: Between 250–500, depending on cell type and format.
Q: Is it compatible with serum?
A: Yes, CANFAST™ works efficiently in complete media.
Q: Can I use it for co-transfection?
A: Yes, it supports co-delivery of multiple plasmids.