Caspase 5 (Caspase-5, CASP 5, CASP5, CASP-5, ICERELIII, ICErel-III, Ich3, Ich-3 Protease, TY, TY Protease)
Cat# C2087-24Y-100ug
Size : 100ug
Marca : US Biological
Host
rabbitIsotype
IgGGrade
Affinity PurifiedApplications
WBShipping Temp
Blue IceStorage Temp
-20°CCaspase-5, a 47kD cysteine protease, otherwise known as ICH-3. Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICEtype) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-2ug/ml, detects a band of ~48kD in Ramos cell lysates. (Predicted precursor ~47.8kD)
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Pab
Isotype
IgG
Host
rabbit
Source
human
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by affinity chromatography.
Immunogen
Synthetic peptide corresponding to 14aa from the center of human Caspase-5.
Specificity
Recognizes human Caspase-5.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.