Caspase 5, ID (Caspase-5, CASP 5, CASP5, CASP-5, ICERELIII, ICErel-III, Ich3, Ich-3 Protease, TY, TY Protease)

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18. Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1b. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Ramos Cell Lysate or P815 Cell Lysate

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

rabbit

human

~1mg/ml

Supplied as a liquid in PBS, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Synthetic peptide corresponding to 14aa from human Caspase-5.

Recognizes human Caspase-5.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.