Catalase Activity Assay Kit
Cat# CA063
Size : 100assays
Marca : Canvax Biotech
Catalase Activity Assay Kit
Fast and sensitive catalase activity detection by colorimetric or fluorometric assay.
The Catalase Activity Assay Kit provides a fast, easy, and highly sensitive method for detecting catalase activity in biological samples. Catalase catalyzes the decomposition of hydrogen peroxide (H₂O₂) into water and oxygen. The assay quantifies unconverted H₂O₂ via either a fluorometric method (Ex/Em = 535/587 nm) or a colorimetric method (570 nm).
The ready-to-use kit includes all reagents and positive controls, with a protocol completed in just 34 minutes. It is ideal for oxidative stress research, antioxidant screening, and enzymatic activity studies.
Have questions? Contact us
Detailed information:
Advantages & Features
-
Protocol completed in ~34 minutes.
-
Dual detection: colorimetric (570 nm) & fluorometric (Ex/Em 535/587 nm).
-
Sensitive and reproducible results.
-
Includes positive control for validation.
-
Suitable for a wide range of biological samples.
Specifications
| Specification | Description |
|---|---|
| Detection | Colorimetric (570 nm) & Fluorometric (Ex/Em 535/587 nm) |
| Assay time | ~34 minutes |
| Includes | Probe, buffers, HRP, H₂O₂, stop solution, positive control |
| Sample types | Cells, tissues, biological extracts |
| Intended use | Research Use Only |
Includes
– 200 μL Probe (in DMSO)
– 20 mL CAT Assay Buffer
– 50 μL H2O2 (8.8 M)
– 250 μL HRP Solution
– 1.5 mL Stop Solution
– 5 mg Catalase Positive Control
Download documentation
Applications
-
Measurement of catalase activity in cell/tissue samples.
-
Oxidative stress research.
-
Antioxidant screening.
-
Drug discovery & biochemical assays.
Tables & Figures
t
Quality Control
-
Strict quality controls performed in every production phase.
-
Each batch validated with positive control assays.
Advice
-
Protect all kit components from light.
-
Perform assays in duplicates or triplicates for higher reproducibility.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 ºC.
Citations
- Shailajan, S., Menon, S., Singh, S., & Patil, Y. (2023). A novel herbal combination ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with letrozole-induced polycystic ovary syndrome. Asian Pacific Journal of Reproduction, 12(1), 23.
- Morcillo Benlloch, M. Á. (2021). Aplicación de la embriogénesis somática y el” priming” para mejorar la tolerancia a Phytophthora cinnamomi en Quercus ilex L.
- Bobadilla, Miriam, et al. “A Grape Juice Supplemented with Natural Grape Extracts Is Well Accepted by Consumers and Reduces Brain Oxidative Stress.” Antioxidants 10.5 (2021): 677.
- Lima-Cabello, E., Morales-Santana, S., León, J., Alché, V., Clemente, A., de Dios Alche, J., & Jimenez-Lopez, J. C. (2018). Narrow-leafed lupin (Lupinus angustifolius L.) seed β-conglutins reverse back the induced insulin resistance in pancreatic cells. Food & Function.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Catalase assay kit, Catalase activity measurement assay, Hydrogen peroxide decomposition assay, Catalase enzyme activity kit, Catalase detection assay
Also known as:
- Spanish: Kit de ensayo de catalasa, Medición de actividad catalasa, Ensayo de descomposición de peróxido de hidrógeno
- French: Kit d’analyse de catalase, Mesure de l’activité catalase, Test de décomposition du peroxyde d’hydrogène
- German: Katalase-Assaykit, Messung der Katalaseaktivität, Wasserstoffperoxid-Zersetzungsassay
- Italian: Kit saggio catalasi, Misurazione attività catalasi, Saggio decomposizione perossido di idrogeno
FAQs
Q: How long does the assay take?
A: Around 34 minutes.
Q: What detection modes are available?
A: Colorimetric (570 nm) and fluorometric (535/587 nm).
Q: Can I use less than 0.1 g of tissue for the assay?
A: Yes, smaller amounts can be used if the resulting activity remains within the assay’s detection range.
Q: How should the protocol be adapted for low tissue input?
A: Reduce the homogenization volume proportionally and verify that the signal remains within the linear range.