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> CD146/MCAM Protein, Human, Recombinant (His)

CD146/MCAM Protein, Human, Recombinant (His)

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Cat# NB-64-56206-100ug

Size : 100ug

Marca : Neo Biotech


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CD146/MCAM Protein, Human, Recombinant (His)

(Synonyms: MUC18, melanoma cell adhesion molecule, CD146) Copy Product Info

Synonyms: MUC18, melanoma cell adhesion molecule, CD146

Catalog No. TMPY-01034 Copy Product Info
CD146/MCAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.8 kDa and the accession number is A0A024R3I5.
Other Forms of CD146/MCAM Protein, Human, Recombinant (His):
CD146/MCAM Protein, Human, Recombinant (hFc)
CD146/MCAM Protein, Mouse, Recombinant (His)
CD146/MCAM Protein, Human, Recombinant (His), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Immobilized Human MUC18, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3 ng/ml determined by ELISA.
Description
CD146/MCAM Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.8 kDa and the accession number is A0A024R3I5.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP43121
ConstructionVal24-Gly559
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsMUC18, melanoma cell adhesion molecule, CD146
Research Background
METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.
Chemical Properties
Molecular Weight60.8 kDa (Predicted); 80-110 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

MUC-18MUC 18CD-146CD 146
Related Tags: CD146/MCAM Protein, Human, Recombinant (His) chemical structure | CD146/MCAM Protein, Human, Recombinant (His) molecular weight