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> CD35 / CR1(CR1/802), CF568 conjugate, 0.1mg/mL

CD35 / CR1(CR1/802), CF568 conjugate, 0.1mg/mL

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Cat# BNC680802-500

Size : 500uL

Marca : Biotium

CD35 / CR1(CR1/802), CF568 conjugate, 0.1mg/mL

CD35 / CR1 Monoclonal Mouse Antibody (CR1/802)

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Monoclonal anti CD35 / CR1 (CR1/802)Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Monoclonal Antibody (CR1/802).
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Product Description

Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21 /CD35 /CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email [email protected] to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0802, Anti-CD35 (CR1/802) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
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CF® Dyes
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Considerations for Immunofluorescence Staining
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Primary & Secondary Antibody Conjugates

Product Attributes

Antibody number
#0802
Antibody reactivity (target)
CD35, CR1
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal
Clone
CR1/802
Isotype
IgG1, kappa
Molecular weight
210-220 kDa
Synonyms
C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen
Human gene symbol
CR1
Entrez gene ID
1378
SwissProt
P17927
Unigene
334019
Immunogen
Recombinant human CR1 protein
Verified antibody applications
IHC (FFPE) (verified)
Antibody target cellular localization
Plasma membrane
Species reactivity
Baboon, Human, Monkey
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user
Positive control
Follicular dendritic cells (FDC) in tonsil
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas
Immunology
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Antigen presenting cells

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