Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I)

Stampa

Cat# C2398-01P-100ug

Size : 100ug

Marca : US Biological

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C2398-01P CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I)

Clone Type

Monoclonal

Host

mouse

Source

equine

Swiss Prot

Q05078

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IHC

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD44 is a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion.

Equine CD44 is widely expressed and C2398-01P may be used as a pan equine leucocyte marker.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat; 1:25-1:200, 10ul labels 10e6 cells in 100ul
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag 8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CVS18|Host: mouse|Source: equine|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide. |Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Equine leukocytes.|Specificity: Recognizes equine CD44. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Equine leukocytes.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes equine CD44.

References

1. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991. Vet Immunol Immunopathol. 42 (1): 3-60.|2. Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1 J Gen Virol. 84: 293-300|3. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 81: 312-23.|4. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations. Stem Cells Dev. 19: 269-82.|5. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cell. Cell Med. 4(1): 1-11.|6. Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses. BMC Vet Res. 11(1): 361.|7. Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow. Microsc Res Tech. 76 (6): 618-24.|8. Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response. Vaccine. 21 (21-22): 3081-92.|9. Tessier L et al. (2015) Phenotypic and immunomodulatory properties of equine cord bloodderived mesenchymal stromal cells. PLoS One. 10 (4): e0122954.|10. Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants. Cell Physiol Biochem. 37 (2): 651-665.|11. Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology. Vet Res Commun. 40 (1): 39-48.|12. Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype. Stem Cell Rev. 12 (2): 245-56.|13. Alvarenga, M.A. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares J Eq Vet Sci. 42: 12-8.|14. Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells. Stem Cells Dev. 25 (1): 80-9.|15. Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells. Cell Biol Int. Nov 26. [Epub ahead of print]|16. Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells. Front Immunol. 7: 392.|17. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord. Theriogenology. 100: 8-15.|18. Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells. Stem Cell Res Ther. 8 (1): 166.|19. Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses. BMC Vet Res. 11: 63.