CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I)

CD44 is a 90kD type I transmembrane protein, otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): 1:100
Immunohistochemistry (Paraffin): 1:100; Requires antigen retrieval using heat treatment with sodium citrate buffer, pH 6 prior to staining of paraffin sections.
Western Blot: Detects a band at ~80kD in HeLa cell lysate.
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-83P1: IgG2a Murine Negative Control

Immunohistochemistry Positive Control:
Human Tonstil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.