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CD8 (PE)

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Cat# C2258-91J-100T

Size : 100Tests

Marca : US Biological


C2258-91J CD8 (PE)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-84B2 IgG2a NEGATIVE CONTROL, Murine (PE)

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. DO NOT FREEZE! For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: CC63|Host: mouse|Source: bovine|Concentration: Not Determined|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile dH2O.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD8. Species Crossreactivity: goat and sheep.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile dH2O.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD8. Species Crossreactivity: goat and sheep.
References
1. MacHugh, N.D. et al. (1991) Individual antigens of cattle. Bovine CD8 (BoCD8). Vet. Immunol. Immunopathol. 27: 65-69. 2. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334.|3. Twizere, J. et al. (2000) Discordance between bovine leukemia virus tax immortalization in vitro and oncogenicity in vivo. J. Virol. 74 (21): 9895 - 9902.