CD8a (Ly2, T cell Co-receptor, Leu2, T8) (FITC)

Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8alpha/alpha homodimer or a CD8alpha/beta heterodimer. It is found on most thymocytes, and on the “suppressor/cytotoxic” subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition.

Applications:
• Flow cytometry1-3
• Immunoprecipitation1
• Cytotoxicity assays1,2

Recommended Dilution:
Flow Cytometry: ≤ 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.