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> CSDE1 Protein Lysate

CSDE1 Protein Lysate

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Cat# OCOA11775

Size : 20ug

Marca : Aviva Systems Biology

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    Datasheets/ManualsPrintable datasheet for CSDE1 Protein Lysate (OCOA11775)
    Product Info
    Predicted Species ReactivityHomo sapiens
    Product FormatLyophilized
    HostHEK293T
    Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
    ::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
    Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
    TagC-MYC/DDK
    DescriptionCSDE1 HEK293T cell transient overexpression lysate (as WB positive control)
    Gene SymbolCSDE1
    Gene Full Namecold shock domain containing E1
    Alias Symbolscold shock domain containing E1, RNA binding;cold shock domain-containing protein E1;D1S155E;N-ras upstream gene protein;NRAS-related;Protein UNR;UNR;upstream of NRAS.
    NCBI Gene Id7812
    Protein NameA0A024R0K2, NM_007158, NP_009089, O75534
    Description of TargetCold shock domain containing E1, RNA-binding, isoform CRA_b, Cold shock domain-containing protein E1, cold shock domain-containing protein E1 isoform 2 [Homo sapiens], Homo sapiens cold shock domain containing E1 (CSDE1), transcript variant 2, mRNA
    Uniprot IDhttps://www.uniprot.org/uniprot/O75534
    Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_009089
    Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_007158
    Molecular Weight85.7 kDa