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> DL-Glyceraldehyde [56-82-6]

DL-Glyceraldehyde [56-82-6]

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Entra
Ordine minimo 2

Cat# HY-128748-1mL

Size : 10mM/1mL

Marca : MedChemExpress


Description

DL-Glyceraldehyde is a bioactive substance involved in cellular energy metabolism and a key intermediate in sugar metabolism pathways (such as glycolysis and gluconeogenesis). During glycolysis, DL-Glyceraldehyde is converted by enzymes into other metabolites to provide energy for cells; during gluconeogenesis, DL-Glyceraldehyde participates in the synthesis of glucose as a precursor. In the field of medical research, DL-Glyceraldehyde can be used to study diseases related to sugar metabolism, such as diabetes, tumors, etc[1][2].

In Vitro

DL-Glyceraldehyde (5 mM; 24 h) can inhibit cell migration and cell viability, arrest the cell cycle at the G0/G1 phase, and induce cell apoptosis[1].
DL-Glyceraldehyde (5 mM; 24 h) upregulates the expression of pro-apoptotic proteins and downregulates the expression of anti-apoptotic proteins in WB experiments[1].
DL-Glyceraldehyde can inhibit cell glycolysis and has a more significant inhibitory effect on the growth of neuroblastoma cells than on Chinese hamster ovary K1 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DL-Glyceraldehyde Related Antibodies

Cell Viability Assay[1]

Cell Line: SW480 cells
Concentration: 5 mM
Incubation Time: 24 h
Result: Significantly decreased cell valibility compared with the control group.

Apoptosis Analysis[1]

Cell Line: SW480 cells
Concentration: 5 mM
Incubation Time: 24 h
Result: Significantly increased the apoptosis rate compared with the control group[1].

Cell Cycle Analysis[1]

Cell Line: SW480 cells
Concentration: 5 mM
Incubation Time: 24 h
Result: Arrested the cell cycle at the G0/G1 phase compared with the control group.

Western Blot Analysis[1]

Cell Line: SW480 cells
Concentration: 5 mM
Incubation Time: 24 h
Result: Up-regulated the expression of pro-apoptotic proteins, and down-regulated the expression of anti-apoptotic proteins compared with the control group.
Masse moléculaire

90.08

Formule

C3H6O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=CC(O)CO
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (1110.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 8.75 mg/mL (97.14 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 11.1012 mL 55.5062 mL 111.0124 mL
5 mM 2.2202 mL 11.1012 mL 22.2025 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (27.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Pureté et documentation
Références