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> Dnmt3L Antibody

Dnmt3L Antibody

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Cat# ASM10217-A680-100

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Marca : Abcepta

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  • IHC - Dnmt3L Antibody ASM10217
    Immunohistochemistry analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (ASM10217). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (ASM10217) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Lower layers of the epidermis have positive cell staining in the cytoplasm.
  • WB - Dnmt3L Antibody ASM10217
    Western Blot analysis of Human tissue lysate showing detection of Dnmt3L protein using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (ASM10217). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (ASM10217) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • ICC/IF - Dnmt3L Antibody ASM10217
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dnmt3L Monoclonal Antibody, Clone S117-9 (ASM10217). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Dnmt3L Monoclonal Antibody (ASM10217) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Staining in the nucleus, and accumulated around the nucleus. Clear nuclear demarcations.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, AM
Primary Accession Q9UJW3
Other Accession NP_037501.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse
Clonality Monoclonal
Description Mouse Anti-Human Dnmt3L Monoclonal IgG1
Target/Specificity Detects ~45kDa. No cross-reactivity against DNMT3a or DNMT3B.
Other Names Cytosine 5 methyltransferase 3 like protein antibody, DNA (cytosine 5 ) methyltransferase 3 like antibody, DNA (cytosine-5)-methyltransferase 3-like antibody, DNA cytosine 5 methyltransferase 3 like protein antibody, DNA methyltransferase 3 like protein antibody, DNM3L_HUMAN antibody, Dnmt 3L antibody, Dnmt3l antibody, Human cytosine 5 methyltransferase 3 like protein antibody, MGC1090 antibody
Clone Names S117-9
Immunogen Fusion protein amino acids 1-387 of human DNMT3L
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-341 was sufficient for detection of DNMT3L in 10 µg of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Nucleus