Donkey Anti-Rabbit IgG antibody (Rhodamine)
Cat# GTX26799-1mg
Size : 1mg
Marca : Genetex
Contatta il distributore locale :
Telefono : +1 850 650 7790
Host | Donkey |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, FACS, Dot, ELISA |
Reactivity | Rabbit |
| Package | 1 mg |
Datasheet File View product citations for antibody GTX26799 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | Assay dependent |
| ICC/IF | 1:1000-1:5000 |
| FACS | 1:500-1:2500 |
| Dot | Assay dependent |
| ELISA | 1:10000-1:50000 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl, 1% BSA
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rabbit
Immunogen
Rabbit IgG whole molecule
Target Isotype
IgG
Purification
IgG fraction
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
Conjugation
Rhodamine
Ratio : 2.4 molecules Rhodamine per Donkey IgG molecule.
Ratio : 2.4 molecules Rhodamine per Donkey IgG molecule.
RRID
AB_378891
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Application Reference
Lee JG et al. J Biol Chem 2018; 293 (10) : 3758-3769 Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
Lee JG et al. J Biol Chem 2015; 290 (44) : 26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.
REVIEW
Application Reference
Lee JG et al. J Biol Chem 2018; 293 (10):3758-3769 Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
Lee JG et al. J Biol Chem 2015; 290 (44):26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.
| SDS | |
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| Sodium Azide.pdf | |