Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

DTNB [69-78-3]

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Cat# NB-64-06458-1g

Size : 1g

Marca : Neo Biotech

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DTNB

(Synonyms: Ellman's Reagent) Copy Product Info

Synonyms: Ellman's Reagent

Catalog No. T15177 Copy Product Info

Purity: 99.51%

DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].

Cas No. 69-78-3

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.51%

Appearance:Solid

Color:White to Yellow

Product Introduction

DTNB AI Summary

DTNB inhibits the binding of wild type HIV YU2 gp120 to CD4 with an IC50 value of 3580.0 nM. It also targets human thymidylate kinase, showing an IC50 value of 1530.0 nM in the presence of dTMP substrate using fluorescence analysis after 30 minutes. Additionally, DTNB exhibits inhibitory activity against various exonuclease enzymes, such as Escherichia coli K-12 RNase T exonuclease, Caenorhabditis elegans CRN-4 exonuclease, and Lassa virus NP exonuclease domain, with inhibitory concentrations of 0.1 mM for the first two enzymes and 1.0 mM for the latter. Moreover, the compound displays substrate activity with recombinant Haemophilus influenzae Chloramphenicol nitroreductase expressed in Escherichia coli, with an activity rate of 3.5% measured under specified conditions. This multifaceted profile suggests potential use as a broad-spectrum inhibitor of enzyme activities..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	DTNB (Ellman's Reagent) is used to quantify thiol groups in a sample and measure low-molecular-mass thiols, such as glutathione, in pure solutions and biological samples [such as blood].
Synonyms	Ellman's Reagent

Chemical Properties

Molecular Weight	396.35
Formula	C₁₄H₈N₂O₈S₂
Cas No.	69-78-3
Smiles	C(O)(=O)C1=C(N(=O)=O)C=CC(SSC2=CC(C(O)=O)=C(N(=O)=O)C=C2)=C1
Relative Density.	1.67 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 247.5 mg/mL (624.45 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (6.31 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5230 mL	12.6151 mL	25.2302 mL	126.1511 mL
5 mM	0.5046 mL	2.5230 mL	5.0460 mL	25.2302 mL
10 mM	0.2523 mL	1.2615 mL	2.5230 mL	12.6151 mL
20 mM	0.1262 mL	0.6308 mL	1.2615 mL	6.3076 mL
50 mM	0.0505 mL	0.2523 mL	0.5046 mL	2.5230 mL
100 mM	0.0252 mL	0.1262 mL	0.2523 mL	1.2615 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

InhibitorinhibitDTNB

Related Tags: DTNB chemical structure | DTNB formula | DTNB molecular weight