EasyPure® EndoFree Plasmid MaxiPrep Kit
Cat# EM123-01
Size : 10rxns
Marca : TransGen Biotech
EasyPure® EndoFree Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from≤500 ml(LB) of bacterial culture. The solution contains indicators that can indicate whether the lysis and neutralization are complete through the change of color, so as to ensure the quality of plasmid extraction and visualize the operation. Plasmid purification by the endotoxin removal solution. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection. The kit can be used 10 times based on a single 100 ml to 200 ml bacterial input.
Features
• Operational visualization: solution LB (blue) indicates whether the lysis and neutralization are complete through the
change of color.
• Fast: the whole procedure can be performed in one hour.
• High purity: high-purity, endotoxin-free (<0.1EU/μg) transfection-grade plasmid DNA can be prepared.
• High yield (up to 4 mg nucleic acid load in the purification column).
Storage
at room temperature (15-25 ℃) under dry conditions for one year.
Shipping
at room temperature.
Component | EM123-01(10 rxns) |
Resuspension Buffer (RB) | 120 ml |
Lysis Buffer (LB, Blue) | 120 ml |
Neutralization Buffer 1 (NB1) | 120 ml |
Activation Buffer (AB) | 55 ml |
Endotoxin Removal Buffer (ER) | 33 ml |
Wash Buffer (WB) | 25 ml |
Elution Buffer (EB) | 25 ml |
RNase A (10 mg/ml ) | 1.2 ml |
Maxi-Plasmid Spin Column with Collection Tube | 10 |
Push Filter | 10 |
50 ml Collection Tube | 10 |