Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare

EGFR Protein, Human, Recombinant (His & Avi)

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Cat# NB-64-54171-500ug

Size : 500ug

Marca : Neo Biotech

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EGFR Protein, Human, Recombinant (His & Avi)

(Synonyms: PIG61, NISBD2, mENA, LEGFR, HER1, ERBB1, ErbB, EGFR, EC 2.7.10.1, EC 2.7.10) Copy Product Info

Synonyms: PIG61, NISBD2, mENA, LEGFR, HER1, ERBB1, ErbB, EGFR, EC 2.7.10.1, EC 2.7.10

Catalog No. TMPK-00426 Copy Product Info

EGFR Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Appearance:Solid

Color:White

Product Information

Bioactivity

Bioactivity	1. Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay. 2. Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay.
Description	EGFR Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 71.5 kDa and the accession number is P00533-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P00533-1
Construction	Leu25-Ser645
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	PIG61, NISBD2, mENA, LEGFR, HER1, ERBB1, ErbB, EGFR, EC 2.7.10.1, EC 2.7.10
Research Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Chemical Properties

Molecular Weight	71.5 kDa (predicted). Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

PIG-61PIG 61NISBD-2NISBD 2HER-1HER 1ERBB-1ERBB 1

Related Tags: EGFR Protein, Human, Recombinant (His & Avi) chemical structure | EGFR Protein, Human, Recombinant (His & Avi) molecular weight