ELISA Wash Buffer, 10X

Cat# orb623170-1l

Size : 1l

Marca : Biorbyt

ELISA Wash Buffer, 10X

ELISA Wash Buffer, 10X

SKU: orb623170

Description

ELISA Wash Buffer effectively rinses ELISA plates between reagent addition steps to remove signal-altering debris yet preserve assay components to reduce background and increase signal. Suitable for antibody-sandwich ELISAs and antigen-down ELISAs.

Images & Validation

Application Notes
When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent:, 1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes. 2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes. 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C, protected from light, providing a useful shelf-life of 6-12 months. When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:, 1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer. 2. Filter sterilize into a sterile amber or brown-glass container. 3. Store at 2-8°C. Concentrated HRP conjugate stocks should be stable for up to 18 months.

Key Properties

Storage & Handling

StorageRoom temp
Concentration10 X
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Protocol Information