Eosin Y
Cat# MBS258482-5x500mL
Size : 5x500mL
Marca : MyBiosource
Eosin Y Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258482Hematoxylin and Eosin (H&E) staining is used most commonly in histology. Hematoxylin is a basic dye and stains basophilic structure. Eosin Y is a fluorescent red dye is used to stain cytoplasm, collagen and muscle fiber. Slides stained with hematoxylin and… More details
Product Overview
Online Datasheet in PDFProduct Name
Eosin Y, Reagent
Full Product Name
Eosin Y
Notes
Ready to use counterstain
Product Categories/Family for Eosin Y reagent
IHC Reagents
Applications Tested/Suitable for Eosin Y reagent
Immunohistochemistry (IHC)
Application Notes for Eosin Y reagent
H and E staining: Follow the procedure as outlined for H and E staining. We use the following protocol:
1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections.
2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water.
3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer (AR-6561), PBS or any buffer with pH 7.4-8.
4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin.
5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent.
6. The slide can be mounted with organic mounting medium or our Organo/limonene (AR-6504) mounting medium.
1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections.
2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water.
3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer (AR-6561), PBS or any buffer with pH 7.4-8.
4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin.
5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent.
6. The slide can be mounted with organic mounting medium or our Organo/limonene (AR-6504) mounting medium.
Other Notes
Small volumes of Eosin Y reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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MBS258482 is a reagent targeting Eosin Y.