Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
> Fish immunoglobulin M (IgM) ELISA Kit

Fish immunoglobulin M (IgM) ELISA Kit

Stampa Stampa
Entra

Cat# CSB-E12045Fh-5x96T

Size : 5x96T

Marca : Cusabio


CSB-E12045Fh
Size 96T,5×96T,10×96T

Product Details

Abbreviation
IgM
Species
Fish
Sample Types
serum, plasma
Detection Range
1.25 μg/mL-50 μg/mL
Sensitivity
1.25 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-101  
1:2 Average % 97  
Range % 90-104  
1:4 Average % 95  
Range % 84-105  
1:8 Average % 95  
Range % 91-99  
Recovery
The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-105  
EDTA plasma (n=4) 95 91-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
50 0.122 0.124 0.123    
25 0.213 0.199 0.206    
11.5 0.401 0.427 0.414    
3.75 0.745 0.761 0.753    
1.25 1.375 1.402 1.389    
0 2.541 2.532 2.537    
Materials provided
    • A 96-well Assay plate --The 96-well plate has been pre-coated with goat-anti-rabbit antibody.
    • Standard (6 x 0.5 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated IgM (1 x 6 ml) --Bind to the IgM antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • IgM Antibody (1 x 6 ml) --Interact with the IgM.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Fish IgM ELISA Kit was designed for the quantitative measurement of Fish IgM protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 μg/mL-50 μg/mL and the sensitivity is 1.25 μg/mL.

Citations

Si può anche essere interessati nei seguenti prodotti: