Free Glycerol Assay Kit (Fluorometric)

Background: Glycerol is the backbone of Triglycerides (TAG). Triglycerides are a type of lipid in the blood, serving as an energy source and play a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lypases hydrolyse the triglyceride ester bond, yielding glycerol and free fatty acids in a process called lipolysis. Enterocytes then absorb and repackage the fragments with cholesterol to form chylomicrons, a major lipoprotein transport particle. In the liver, hepatic lypases also break down triglycerides to assemble another lipoprotein particle (VLDL) from triglycerides, cholesterol, and apolipoproteins. Free Glycerol Assay Kit measures free, endogenous glycerol by a coupled enzymatic reaction system. The glycerol is phosphorylated and oxidized, producing hydrogen peroxide which reacts with the kit's Fluorometric Probe (Ex. 530-560 nm/Em. 585-595 nm). The Free Glycerol Assay Kit is a simple, fluorometric assay that quantitatively measures the amount of glycerol in plasma or serum in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, glycerol standards and unknown samples. The kit contains a glycerol standard and has a detection sensitivity limit of ~1 muM (0.009 mg/dL).

1. Bortz, W.M., Paul, P., Hff, A.C., and Holmes, W.H. (1972) J. Clin. Invest. 51, 1537-1546.
2. Arner, P. (1995) Internation J. of Obesity and Related Metabolic Disorders 19(7), 435-442.
3. Ou, C-N and Frawley, V.L. (1985) Clin. Biochem. 18(1), 37-39.
4. Bernert, J.T., Bell, C.J., McGuffey, J.E., and Waymack, P.P. (1992) J. Chromatogr. 578, 1-7.

Small volumes of Free Glycerol assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168347 is an assay kit targeting Free Glycerol. Also known as Free Glycerol Assay Kit (Fluorometric).