Hexokinase Colorimetric Assay Kit

Hexokinases phosphorylates glucose to generate glucose-6-phosphate, the initial step in glucose metabolism. HK1 and HK2 are two subtypes of mitochondrial hexokinases which are also involved in antiapopotic and cell survival. Hexokinase deficiency leads to X-linked muscular dystrophy and autosomal recessive hemolytic anemia. Increased hexokinase activity is detected in tumors and associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. In this Hexokinase Colorimetric Assay Kit, hexokinase converts glucose into glucose-6-phosphate which is further oxidized by glucose-6-phosphate dehydrogenase to form NADH, and in turn reduces a colorless probe to a colored product with intense absorbance at 450 nm. The assay offers an easy and sensitive way for measuring hexokinase activity. The detection limit can be as low as 0.1 mU/well.