Avidin and streptavidin amplifications systems for Western blot and ELISA. May be used in place of whole antiserum in almost any appropriate antibody based technique.
Reactivity
Human Immunodeficiency Virus 1
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Purified native gp120 from strain IIIB.
Specificity
Recognizes gp120 (predominate gp120 band in Western blot). Antiserum does not cross-react with Human T or B cells. Antiserum is not reactive with Human or Bovine sera.
Buffer
0.01M PBS, pH 7.2 Label: Biotin State: Liquid purified IgG fraction Stabilizer: None Preservative: 0.09% Sodium Azide Label: Covalently coupled with the N-Hydroxysuccinimide ester of under mild conditions to give a high degree of substitution
Concentration
lot specific
Conjugation
Biotin
Storage
Store undiluted at 2-8°C for up to six months or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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