HK-2xZFN-Smc4-mEGFP Clone 82-68
Marca : CLS Cell Lines Service
HK-2xZFN-Smc4-mEGFP Clone 82-68 Cells
General information
Description | The HK-2xZFN-Smc4-mEGFP Clone 82-68 cell line is a genetically engineered derivative of the human kidney proximal tubule epithelial cell line, HK-2. This cell line has been modified using zinc-finger nuclease (ZFN) technology to incorporate a fluorescently tagged version of the structural maintenance of chromosomes 4 (Smc4) protein, fused to the monomeric enhanced green fluorescent protein (mEGFP). The Smc4 protein is a critical component of the condensin complex, which plays a crucial role in chromosome condensation and segregation during cell division. The addition of mEGFP allows for real-time visualization and tracking of Smc4 dynamics within live cells, facilitating studies on chromosome architecture and the mechanisms governing mitosis. The HK-2xZFN-Smc4-mEGFP Clone 82-68 cell line is particularly valuable for research focused on cell cycle regulation, chromosomal behavior, and the structural maintenance of chromosomes. The stable expression of the mEGFP-tagged Smc4 protein ensures consistent fluorescence, making this cell line an excellent tool for live-cell imaging and quantitative analysis of condensin complex functions. Furthermore, the use of proximal tubule epithelial cells as the parental line provides a relevant model for kidney-specific studies, including those related to renal physiology, pathology, and the impact of genetic modifications on kidney function and cellular processes. Overall, the HK-2xZFN-Smc4-mEGFP Clone 82-68 cell line serves as a powerful and versatile model for investigating the fundamental aspects of chromosome biology and kidney cell function, offering insights that are crucial for both basic research and potential clinical applications in nephrology and related fields. |
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Organism | Human |
Tissue | Endocervix |
Disease | Adenocarcinoma |
Characteristics
Age | 30 years |
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Gender | Female |
Ethnicity | African American |
Morphology | Epithelial-like cells with mosaic stone shape |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HK-2xZFN-Smc4-mEGFP Clone 82-68 (Cytion catalog number 301576) |
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Biosafety level | 1 |
Depositor | Dr. J. Ellenberg, EMBL Heidelberg |
Expression / Mutation
Products | EGFP (Enhanced Green Fluorescent Protein) |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 in the beginning and up to 1:15 after establishment of the culture is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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