ASM10309-A655-100 | HSF1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:2000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of ~85 and 95 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Lane 1: Molecular Weight Ladder. Lane 2: A431 cell lysates. Lane 3: HEK293 cell lysates. Load: 20 µg. Block: 5% milk + TBST 1hr at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rat: HRP at 1:1000 for 60 min at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~85 and 95 kDa.
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 4B4 (ASM10309). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10309) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon activation. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF, IP, E, GS
Primary Accession	P38532
Other Accession	NP_032322.1
Host	Rat
Isotype	IgG1
Reactivity	Human, Mouse, Rat, Rabbit, Hamster, Monkey, Bovine, Guinea Pig
Clonality	Monoclonal
Description	Rat Anti-Mouse HSF1 Monoclonal IgG1
Target/Specificity	Detects ~85kDa (unstressed cell lysates) and ~95kDa (heat shocked cell lysates).
Other Names	HSTF1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody
Clone Names	4B4
Immunogen	Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	1 µg/ml of SMC-477 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.
Cellular Localization	Cytoplasm \| Nucleus

HSF1 Antibody

Cat# ASM10309-A655-100

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