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> HSP40, Hdj1 Antibody

HSP40, Hdj1 Antibody

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Cat# ASM10065-STR-100

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Marca : Abcepta

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  • ICC/IF - HSP40, Hdj1 Antibody ASM10065
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (ASM10065). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (ASM10065) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
  • WB - HSP40, Hdj1 Antibody ASM10065
    Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6 (ASM10065). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (ASM10065) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, IP, E
Primary Accession P25685
Other Accession NP_006136.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Human HSP40, Hdj1 Monoclonal IgG1
Target/Specificity Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
Other Names DNAJ1 Antibody, NDAJB1 Antibody, HDJ1 Antibody, HSP40 Antibody, HSPF1 Antibody, DnaJ homolog subfamily B member 1 Antibody, Dna J protein homolog 1 Antibody, Heat shock 40 kDa protein 1 Antibody, HSP40 Antibody, heat shock protein 40 Antibody, Human DnaJ protein 1 Antibody, hDj-1 Antibody
Clone Names 3B9.E6
Immunogen Recombinant Protein HSP40 (Hdj1)
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Nucleus