RayBio® Human CD40 Ligand (TNFSF5) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human CD40L ELISA Kit
Human CD40L ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 6 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 6 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 3 fold |
| Accession Number | P29965 |
| Gene Id | 959 |
| Gene Symbols | CD40LG, CD40L, TNFSF5, TRAP |
| Protein Name / Synonyms | CD40 ligand (CD40-L) (T-cell antigen Gp39) (TNF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD antigen CD154) [Cleaved into: CD40 ligand, membrane form CD40 ligand, soluble form] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human CD40L ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified CD40 Ligand (TNFSF5 / CD40LG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Comparative Effects of Efavirenz and Dolutegravir on Metabolomic and Inflammatory Profiles, and Platelet Activation of People Living with HIV: A Pilot Study
- Species:
- Human
- Sample Type:
- Plasma (people living with HIV and HIV-uninfected controls)
- Year:
- 2024
- Journal Name:
- Viruses
- PubMed ID:
- 39339938
Combination Therapy of Novel Oncolytic Adenovirus with Anti-PD1 Resulted in Enhanced Anti-Cancer Effect in Syngeneic Immunocompetent Melanoma Mouse Model
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of MUG Mel-1, MUG Mel-2 cells)
- Year:
- 2021
- Journal Name:
- Pharmaceutics
- PubMed ID:
- 33919827
Systemic immunity upon local oncolytic virotherapy armed with immunostimulatory genes may be supported by tumor-derived exosomes
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (exosomes isolated from Mel526 cells)
- Year:
- 2021
- Journal Name:
- Molecular Therapy-Oncolytics
- PubMed ID:
- 33738337
Immune response is an important aspect of the antitumor effect produced by a CD40L-encoding oncolytic adenovirus
- Species:
- Human
- Sample Type:
- Serum (Adenovirus expressed Human CD40L)
- Year:
- 2012
- Journal Name:
- Cancer Research
- PubMed ID:
- 22396493
Investigating soluble CD40 ligand as a prognostic factor among acute coronary syndromes patients: A multi-center prospective case-control study
- Species:
- Human
- Sample Type:
- Serum (acute coronary syndrome patients, ICU)
- Year:
- 2024
- Journal Name:
- Medicine
- PubMed ID:
- 39331861
Self complementary adeno associated virus 5 mediated gene transduction of a novel CD40L mutant confers direct antitumor effects in lung carcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (Adenovirus 5 mediated gene transduction); Serum (Adenovirus 5 mediated gene transduction)
- Year:
- 2014
