General Info
| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13~5000pg/mL |
| Short Description: | This human FABP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | FABP4 |
| Gene ID: | 2167 |
| Uniprot ID: | FABP4_HUMAN |
| Specificity: | This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. |
| Protein Name | Fatty Acid-Binding Protein - AdipocyteAdipocyte Lipid-Binding ProteinAlbpAdipocyte-Type Fatty Acid-Binding ProteinA-FabpAfabpFatty Acid-Binding Protein 4 |
| Database Links | Reactome: R-HSA-163560Reactome: R-HSA-381340 |
| Cellular Localisation | CytoplasmNucleusDepending On The Nature Of The LigandA Conformation Change Exposes A Nuclear Localization Motif And The Protein Is Transported Into The NucleusSubject To Constitutive Nuclear Export |
| Alternative CLIA Names | Fatty Acid-Binding Protein - Adipocyte CLIA kitAdipocyte Lipid-Binding Protein CLIA kitAlbp CLIA kitAdipocyte-Type Fatty Acid-Binding Protein CLIA kitA-Fabp CLIA kitAfabp CLIA kitFatty Acid-Binding Protein 4 CLIA kitFABP4 CLIA kit |
| Specificity | This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-101 | 93 |
| EDTA plasma(n=8) | 101-118 | 108 |
| Cell culture media(n=8) | 85-98 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 231.78 | 499.34 | 1814.16 | 228.49 | 518.13 | 1728.90 |
| Standard deviation | 23.66 | 48.74 | 170.89 | 26.73 | 50.10 | 158.71 |
| CV (%) | 10.21 | 9.76 | 9.42 | 11.70 | 9.67 | 9.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance