Human FABP5 (Fatty acid-binding protein 5) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FABP5
Gene ID:	2171
Uniprot ID:	FABP5_HUMAN
Specificity:	This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Keratinocytes.highly expressed in psoriatic skin. Expressed in brain gray matter.
Function	Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors. Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta.which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation. May be involved in keratinocyte differentiation.
Protein Name	Fatty Acid-Binding Protein 5 Epidermal-Type Fatty Acid-Binding Protein E-Fabp Fatty Acid-Binding Protein - Epidermal Psoriasis-Associated Fatty Acid-Binding Protein Homolog Pa-Fabp
Database Links	Reactome: R-HSA-163560 Reactome: R-HSA-5362517 Reactome: R-HSA-6798695
Cellular Localisation	Cytoplasm Nucleus Synapse Postsynaptic Density Secreted Localizes Primarily To The Cytoplasm Upon Certain Ligand Binding A Conformation Change Exposes A Nuclear Localization Motif And The Protein Is Transported Into Nucleus Secreted By Astrocytes But Not By Neurons
Alternative CLIA Names	Fatty Acid-Binding Protein 5 CLIA kit Epidermal-Type Fatty Acid-Binding Protein CLIA kit E-Fabp CLIA kit Fatty Acid-Binding Protein - Epidermal CLIA kit Psoriasis-Associated Fatty Acid-Binding Protein Homolog CLIA kit Pa-Fabp CLIA kit FABP5 CLIA kit
Specificity	This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	100-117	106
EDTA plasma(n=8)	96-108	102
Cell culture media(n=8)	85-97	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	205.65	640.90	1925.48	216.47	642.07	2075.49
Standard deviation	25.77	73.00	203.72	27.15	73.07	130.34
CV (%)	12.53	11.39	10.58	12.54	11.38	6.28

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance