Human Fibronectin
Cat# pro-448-200ug
Size : 200ug
Marca : Prospec
Catalogue number
PRO-448
Introduction
Description
Human Fibronectin produced purified from Human Plasma having a Molecular Weight of 440kDa.
Source
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
The Fibronectin was lyophilized from a non sterile 2mg/ml buffer of 10mM sodium phosphate, pH 7.5 and 0.15M NaCl.
Solubility
We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea.
When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
Stability
Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
≥ 95.0% as determined by SDS-PAGE.