Human G-CSF Antibody : LE/AF
Cat# OASB01234
Size : 0.5mg
Marca : Aviva Systems Biology
Human G-CSF Antibody : LE/AF (OASB01234)
| Datasheets/Manuals | Printable datasheet for Human G-CSF Antibody : LE/AF (OASB01234) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | BVD13-3A5 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | IHC-F, ICC |
| Reconstitution and Storage | Store at -20C |
| Immunogen | E. coli-expressed human G-CSF |
| Concentration | 0.5 mg/mL |
| Specificity | G-CSF |
| Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform tocharacteristics of a standard reference reagent. |
| Application Info | ELISA Capture: BVD13-3A5 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying human G-CSF protein levels. 1, 2 Purified BVD13-3A5 antibody should be paired with biotinylated BVD11-37G10 antibody (OASB01236) as the detection antibody, with purified recombinant human G-CSF as the standard. Western Blotting: The purified BVD13-3A5 antibody is also useful for Western blotting applications. A concentration of 1-5ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant human G-CSF under reducing conditions. Neutralization: The LE/AF form of BVD13-3A5 can be used for neutralizing human G-CSF bioactivity. A suitable LE/AF Rat IgG1 (OASB00095) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti- cytokine antibodies. In CurrentProtocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit6.20 2. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti- cytokine monoclonalantibody development: Immunoassay of IL- 10 and IL- 5 in clinical samples. Immunol. Rev. 127:5- 24. |
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| Gene Symbol | CSF3 |
| Gene Full Name | Granulocyte colony-stimulating factor |
| Alias Symbols | GCSF, CSF3OS, C17orf33 |
| NCBI Gene Id | 1440 |
| Protein Name | granulocyte colony-stimulating factor |
| Description of Target | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. |
| Uniprot ID | P09919 |
| Protein Accession # | NP_000750.1 |
| Nucleotide Accession # | NM_000759.3 |
