RayBio® Human GRO alpha (CXCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human GRO-alpha ELISA Kit
Human GRO-alpha ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09341 |
| Gene Id | 2919 |
| Gene Symbols | CXCL1, GRO, GRO1, GROA, MGSA, SCYB1 |
| Protein Name / Synonyms | Growth-regulated alpha protein (C-X-C motif chemokine 1) (GRO-alpha(1-73)) (Melanoma growth stimulatory activity) (MGSA) (Neutrophil-activating protein 3) (NAP-3) [Cleaved into: GRO-alpha(4-73) GRO-alpha(5-73) GRO-alpha(6-73)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human GRO-alpha ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified CXCL1 (GRO alpha / KC / CINC-1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Chemotaxis-driven delivery of nano-pathogenoids for complete eradication of tumors post-phototherapy
- Species:
- Mouse
- Sample Type:
- Tissue Lysate
- Year:
- 2020
- Journal Name:
- Nature Communications
- PubMed ID:
- 32111847
Immediate Pre-Partum SARS-CoV-2 Status and Immune Profiling of Breastmilk: A Case-Control Study
- Species:
- Human
- Sample Type:
- Other Body Fluid (breastmilk)
- Year:
- 2021
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 34381462
Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
- Species:
- Human
- Sample Type:
- Urine (urine clean-catch midstream)
- Year:
- 2021
- Journal Name:
- Frontiers in Cellular and Infection Microbiology
- PubMed ID:
- 34660348
LAMC1 upregulation via TGFβ induces inflammatory cancer-associated fibroblasts in esophageal squamous cell carcinoma via NF-κB-CXCL1-STAT3
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media CM of cultured cells)
- Year:
- 2021
- Journal Name:
- Molecular Oncology
- PubMed ID:
- 34218518
Cytokines CCL2 and CXCL1 may be potential novel predictors of early bone loss
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- Molecular Medicine Reports
- PubMed ID:
- 33173955
Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care
- Species:
- Human
- Sample Type:
- Other / Unspecified (PRP releasate)
- Year:
- 2020
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 31963580
Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression
- Species:
- Human
- Sample Type:
- Conditioned Media (Fallopian Tube Secretory Epithelial Cells)
- Year:
- 2016
- Journal Name:
- Oncogene
- PubMed ID:
- 26657155
Angiogenic and innate immune responses triggered by PRP in tendon cells are not modified by hyperuricemia
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (tendon cell supernatants)
- Year:
- 2019
- Journal Name:
- Muscles Ligaments and Tendons Journal
- PubMed ID:
- 25489545
OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
- Species:
- Human
- Sample Type:
- Conditioned Media (Non-small cell lung cancer)
- Year:
- 2014
- Journal Name:
- Molecular Cancer Therapeutics
Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (PC3, prostate cancer cell lines)
- Year:
- 2012
- Journal Name:
- Molecular Cancer Therapeutics
