RayBio® Human IFN-gamma ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Human IFN-gamma ELISA Kit
Human IFN-gamma ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 15 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 15 pg/ml - 15000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01579 |
| Gene Id | 3458 |
| Gene Symbols | IFNG |
| Protein Name / Synonyms | Interferon gamma (IFN-gamma) (Immune interferon) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Autophagy, Innate Immunity |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Target Background
Interferon-gamma (IFN-gamma) is a pleiotropic cytokine with antiproliferative, pro-apoptotic and antitumor activity. It is a homodimer formed by the association of two 17 kDa polypeptides, that bind in an antiparallel manner to form a mature 50 kDa molecule. This symmetry allows for a single molecule to bind two receptors amplifying downstream signaling. IFN-gamma is secreted by CD4 T helper type 1 cells and CD8 cytotoxic cells, γδ T cells, NK cells and to a lesser extent by NK T cells, B cells and APC cells. Its expression is induced by cytokines such as IL-12, IL-15, IL-18 and type I IFN. The effects of IFN-gamma are mediated through the activation of the JAK/STAT pathway which modulates the transcription of many genes. IFN-gamma signaling plays a role in host defense, immune surveillance, establishing adaptive immunity and in regulating inflammation, apoptosis and the cell cycle.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human IFN-gamma ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IFN-gamma (IFNG), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Differential antiviral effects and immune responses in nasal and airway organoid during RSV infection: implications for interferon therapy
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (RSV-infected nasal/airway organoids)
- Year:
- 2026
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 41789060
Bispecific antibodies cross-link and redirect CD8+ T cells to kill cytomegalovirus-infected cells
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of CD8+ T cells co-cultured with CMV-infected ARPE-19 cells)
- Year:
- 2025
- Journal Name:
- Science Advances
- PubMed ID:
- 40938983
Molecular profiling and functional analyses of umbilical cord mesenchymal stem cell-derived extracellular vesicles for dry eye diseases
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants of PHA-induced PBMCs)
- Year:
- 2026
- Journal Name:
- Extracellular vesicles and circulating nucleic acids
- PubMed ID:
- 42459926
Immunomodulatory Behavior of Tendon Magnetic Cell Sheets can be Modulated in Hypoxic Environments under Magnetic Stimulus
- Year:
- 2024
- Journal Name:
- ACS Applied Materials & Interfaces
- PubMed ID:
- 39143034
Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell supernatants from exhausted CD4+ T cells)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 40790054
The application of HER2 and CD47 CAR-macrophage in ovarian cancer
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2023
- Journal Name:
- Journal of Translational Medicine
The Effects of Smoking on Telomere Length, Induction of Oncogenic Stress, and Chronic Inflammatory Responses Leading to Aging
- Species:
- Human
- Sample Type:
- Plasma (smokers and non-smokers)
- Year:
- 2024
- Journal Name:
- Cells
- PubMed ID:
- 38891017
A Prospective Exploratory Study of Functional Immunity Assessed by Pretreatment Interferon Gamma Release Assay in Relation to Different Systemic Therapies in Patients With Advanced-Stage NSCLC
- Species:
- Human
- Sample Type:
- Other / Unspecified (PHA-coated tube in the QFT-GIT kit)
- Year:
- 2025
- Journal Name:
- JTO clinical and research reports
- PubMed ID:
- 40612587
Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression
- Species:
- Human
- Sample Type:
- head and neck squamous cell carcinoma (HNSCC) tissue, non-small cell lung cancer (NSCLC) tissue
- Year:
- 2022
- Journal Name:
- Nature Communications
Early-diagnosis of major depressive disorder: From biomarkers to point-of-care testing
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Trac-trends in Analytical Chemistry